When: February 17, 2016, 2:00 PM
Location: 3rd Floor Orchard View Room , Discovery Building
Contact: 608-316-4401, email@example.com
Integrative computational approaches to studying mammalian gene regulatory networks
An emerging challenge in infectious disease research is to integrate diverse types of functional genomics datasets to identify important gene and pathway-level drivers of mammalian host response. We present a computational approach based on a predictive regulatory network model to integrate host mRNA levels, protein levels and protein-protein interactions and to identify important regulators of host response to pathogenic infections. We demonstrate our approach in an application to host response to multiple Influenza A viruses in two study systems: human Calu-3 cell line and mouse lung. We inferred integrated regulatory programs for modules that capture strain- and pathogenicity-specific patterns of immune response. RNAi-based validation of predicted regulators identified several with a significant impact on viral replication, demonstrating the potential of our approach to guide experimentation. Our method is generally applicable to integrate multi-level omics data to gain gene, module and pathway level understanding of regulatory networks.
All QBio sponsored talks take place on Wednesdays at 2:00 p.m. in the 3rd floor Orchard View room of the Discovery Building. Talks are open to the public. Access to the room is via the elevator behind Aldo’s Cafe in the Northeast corner of the building.