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Qbio Seminar Series: Jan Huisken

Event Details

When: September 28, 2016, 2:00 PM

Location: Researchers' Link, 2nd floor of Discovery building, Discovery Building

Contact: 608-316-4401, hstampfli@wisc.edu

Jan Huisken


Jan Huisken
Director, Medical Engineering
Morgridge Institute for Research

Real-time developmental biology by smart and gentle light sheet microscopy

Light sheet microscopy has become the tool of choice for in vivo fluorescence imaging of fragile biological specimens. Low phototoxicity and high speed acquisition have offered unique applications ranging from fast volumetric imaging to extended timelapse experiments spanning the entire embryogenesis of fish and fly. The enormous amounts of data from these instruments (TB/hour) now require a new approach to imaging: we cannot blindly image large samples with uniformly high spatial and temporal resolution.

We propose smart microscopy as a solution, in which illumination and detection are adaptively adjusted to obtain meaningful information at any time without any a priori knowledge about the sample. Only the regions in the sample that change over time or exhibit interesting new information will be illuminated and detected. Alternative modalities such as optical projection tomography offer additional information about the location and state of the sample that are used to optimize light sheet acquisitions. This presentation will give an overview of the latest developments in light sheet microscopy that lead towards a smart and gentle microscope.

   

All QBio sponsored talks take place on Wednesdays at 2:00 p.m. in the 3rd floor Orchard View room of the Discovery Building unless otherwise noted. Talks are open to the public. Access to the room is via the elevator behind Aldo’s Cafe in the Northeast corner of the building.