Sridharan Lab

 

2016 Sridharah Lab MembersLab leader: Rupa Sridharan

Assistant Professor of Cell and Regenerative Biology

Research Interests

Epigenetics of cell fate change

Embryonic stem (ES) cells have the ability to divide indefinitely and to differentiate into any tissue under the correct set of chemical stimuli. Transcription factor-mediated reprogramming, initially demonstrated in mouse somatic cells, is the process by which the overexpression of a few transcription factors, usually, Oct4, Sox2, c-Myc and Klf4, converts differentiated cells into induced pluripotent stem (iPS) cells. Multiple molecular and functional studies have shown that iPS cells are highly similar to ES cells. Human somatic cells can also be reprogrammed, providing iPS cells both as tools for translational research such as for in vitro drug screens and for cell replacement therapy. Only about 1 percent of cells complete the reprogramming process, suggesting that multiple barriers have to be overcome for this dramatic change in cell fate to occur. Research in the lab focuses on understanding the epigenetic roadblocks to the reprogramming process to illuminate both the mechanisms that control pluripotency and the stability of the differentiated state.

Specifically, we want to answer the following questions:

  • How do the reprogramming factors activate pluripotency loci?
  • What controls the global chromatin structure during reprogramming?
  • Are there common principles in the reversion of differentiation that can be applied to switching the lineage between two differentiated cell types?

Insights from these basic research studies may enable the rational development of more efficient methods of reprogramming somatic cells for use in therapeutic settings.

 

Recent Publications

Jackson, S.A. *, Olufs, Z.P.G. *, Tran, K.A., Zaidan, N.Z., and Sridharan, R. (2015) Alternative routes to induced pluripotent stem cells revealed by reprogramming of neural lineageStem Cell Reports (in press, available online Feb 18 2016).  * co-first authors.

Current lab members

  • Nicole Adrian

    Nicole Adrian

    Undergraduate Research Assistant, Epigenetics

    • nadrian@wisc.edu
  • Dan Devine

    Daniel Devine

    Research Specialist, Epigenetics

    • ddevine2@wisc.edu
  • Rupa Sridharan

    Rupa Sridharan

    Assistant Professor, Cell and Regenerative Biology, Epigenetics

    • rsridharan@discovery.wisc.edu
    • 608-316-4422
  • Khoa Tran

    Khoa Tran

    Graduate Student, Epigenetics

    • katran@wisc.edu
  • Coral Wille

    Coral Wille

    Postdoctoral Fellow, Epigenetics

    • ckwille@wisc.edu
  • Amber Zhou

    Amber Zhou

    Graduate Student, Epigenetics

    • aszhou@wisc.edu

 

Current Opportunities

The Sridharan Lab is seeking a strong candidate for the position of postdoctoral fellow. Please contact Rupa Sridharan.