Featured event

biological discovery

SysBioM Lecture Series – Josh Coon

Event Details

When: November 11, 2015, 2:00 PM

Location: 3rd Floor Orchard View Room , Discovery Building

Contact: 608-316-4401, hstampfli@wisc.edu

One Hour Proteomes and Deep Proteome Sequencing

Josh Coon

Josh Coon

Modern biological and medicinal researchers increasingly rely on technologies for rapid and large-scale the comparative analysis of proteomes. Here we describe the identification of ~4,000 yeast proteins, a comprehensive yeast proteome, from just over an hour of analysis time. We achieved this expedited proteome characterization through improved sample preparation, chromatographic separations, and by using a new Orbitrap hybrid mass spectrometer. Next we applied this technology to characterize over 1,000 yeast proteomes in a high-throughput analysis to infer gene function. Coupling this technology with a multi-enzyme digestion approach and multiple MS/MS dissociation techniques (i.e., ETD, HCD, CAD), we also report the deepest coverage achieved to date of the yeast proteome. In this work we detect 5,033 yeast proteins (detection of 3 dubious ORFs, two of which had very high sequence coverage) and had a median sequence coverage of > 80%. Specifically, we recorded mass spectral evidence for 8 of every 10 amino acids in the yeast proteome.

All SysBioM (Systems Biology in Madison) sponsored talks take place on Wednesdays at 2p.m. in the 3rd floor Orchard View room of the Discovery Building. Talks are open to the public. Access to the room is via the elevator behind Aldo’s Cafe in the Northeast corner of the building.