When: March 2, 2016, 2:00 PM
Location: 3rd Floor Orchard View Room , Discovery Building
Contact: 608-316-4401, email@example.com
An insight into interactions between germinating spores of Streptomyces bacteria-signaling within and between species via small molecules
Soil dwelling and filamentous, Streptomyces starts its complex life cycle by germinating from single spores and ventures into vegetative growth of substrate mycelium, from which the aerial hyphae arise, extend, coil and develop into chains of spores. As the first step of the life cycle, germination is the critical decision made by spores of Streptomyces which shifts the bacterium from dormancy to the challenging multicellular life. To understand interactions between germinating spores of Streptomyces as well as physiological and environmental effects on germination, we constructed spores expressing either red or green fluorescence, and implemented devices and scripts for live imaging, measuring and quantitatively analyzing germination process. With the interactive scoring system we set up for characterizing phenotypic varieties and plotting germination curves under different conditions, we captured interesting morphologies developed during the germination and established interaction matrix of germinating spores within and between Streptomyces species: positive interactions could happen between sibling spores by promotive factors released to activate its own germination, while promotion or inhibition could be triggered between germinating spores of different species. Our work brought a quantitative insight into morphological dynamics of Streptomyces germination at population level and provided a thorough grounding for further characterization of the signaling molecules released by germinating spores.
All QBio sponsored talks take place on Wednesdays at 2:00 p.m. in the 3rd floor Orchard View room of the Discovery Building. Talks are open to the public. Access to the room is via the elevator behind Aldo’s Cafe in the Northeast corner of the building.