When: April 26, 2017, 2:00 pm
Location: 3rd Floor Orchard View Room , Discovery Building
Contact: 608-316-4401, email@example.com
Metabolomics in aging and disease
Metabolic rewiring is a key hallmark of several diseases including cancer, stroke, diabetes, and heart disease. During my talk, I will provide two examples of studying metabolism to identify potential biomarkers and identify novel pathways involved in aging and age-related diseases. First study relates to maturation of human pluripotent stem cells derived cardiomyocytes (hPSC-CMs). hPSC-CMs hold immense clinical potential but their use in clinic is limited owing to their immature phenotype and arrhythmogenic nature in vivo, necessitating development of methods to mature these cells. We identified global metabolic changes which take place during long-term culture and maturation of hPSC-CMs derived from three different hPSC lines. We have identified metabolic pathways which showed significant enrichment upon maturation. Understanding the role of these metabolic changes in maturation process has the potential to develop novel approaches to monitor and expedite hPSC-CM maturation.
In my second half of the talk I would share our work (in collaboration with Dr. Wesley, Dept. of Neurosurgery) on studying effects of aging on metabolism in ischemic injury model in rat tissues and serum. Stroke survivors show significant neurological deficits and the severity of the disability correlates with age. Systemic effects of ischemic reperfusion (I/R) injury can cause inflammation in distant tissues. The effects of stroke are partially recovered with time as indicated by improvement in activities of daily living (ADL) function several months after stroke incidence. We examined the dysregulation of metabolites in serum, brain, and liver as a function of age to examine the role of aging and effect on distant organs in a time dependent manner. Biomarker analysis revealed citrate as a putative biomarker of I/R in serum as it was depleted in an age and time independent manner. These data suggest an important role for imbalance in metabolites in stroke pathogenesis.
All QBio sponsored talks take place on Wednesdays at 2:00 p.m. in the 3rd floor Orchard View room of the Discovery Building unless otherwise noted. Talks are open to the public. Access to the room is via the elevator behind Aldo’s Cafe in the Northeast corner of the building.