When: March 18, 2015, 1:00 PM
Location: 1st floor (west end of building by the Entrepreneurial Resource Center), Discovery Building
Cost: Free and open to the public
Contact: 608-316-4325, firstname.lastname@example.org
Lloyd Smith will speak on Building a Genomic Microscope: Hybridization Capture of Chromatin Associated Proteins for Proteomics (HyCCAPP)
DNA−protein interactions play critical roles in the control of genome expression and other fundamental processes. An essential element in understanding how these systems function is to identify their molecular components. We have developed a novel strategy, Hybridization Capture of Chromatin Associated Proteins for Proteomics (HyCCAPP), to identify proteins that are interacting with any given region of the genome (J. Proteome Res. 13 (8), 3810−3825). This technology identifies and quantifies the proteins that are specifically interacting with a genomic region of interest by sequence-specific hybridization capture of the target region from in vivo cross-linked chromatin, followed by mass spectrometric identification and quantification of associated proteins. The approach has been successfully applied to single and multi-copy loci in yeast, as well as to multi-copy loci in human. The strategy, results to date, and interesting associated issues will be presented and discussed.
SysBioM Seminars are sponsored by the Systems Biology Theme in the Wisconsin Institute for Discovery and feature speakers from various disciplines of Systems Biology including, but not limited to Genetics, Biostatistics, Microbiology, and Chemical and Biological Engineering.
The talks are located on the first floor west side of the Discovery Building in the ERC Lobby.
For more information please contact plpointer “at” wisc.edu