About
Recent Publications:
Wille CK, Zhang X, Haws S, Denu J, Sridharan R. Antagonistic H3K79me-H3K9ac crosstalk determines elongation at housekeeping genes to promote pluripotency. bioRxiv 2022.09.26.509534; doi: https://doi.org/10.1101/2022.09.26.509534 Wille CK, Sridharan R. Connecting the DOTs on Cell Identity. Front Cell Dev Biol. 2022;10:906713. doi: 10.3389/fcell.2022.906713. eCollection 2022. Review. PubMed PMID: 35733849; PubMed Central PMCID: PMC9207516. https://doi.org/10.3389/fcell.2022.906713 Wille CK, Sridharan R. DOT1L inhibition enhances pluripotency beyond acquisition of epithelial identity and without immediate suppression of the somatic transcriptome. Stem Cell Reports. 2022 Feb 8;17(2):384-396. doi: 10.1016/j.stemcr.2021.12.004. Epub 2022 Jan 6. PubMed PMID: 34995500; PubMed Central PMCID: PMC8828533. https://doi.org/10.1016/j.stemcr.2021.12.004 Wille CK, Neumann EN, Deshpande AJ, Sridharan R. DOT1L interaction partner AF10 controls patterning of H3K79 methylation to maintain cell identity. bioRxiv 2020.12.17.423347; doi: https://doi.org/10.1101/2020.12.17.423347 Haws SA, Yu D, Ye C, Wille CK, Nguyen LC, Krautkramer KA, Tomasiewicz JL, Yang SE, Miller BR, Liu WH, Igarashi K, Sridharan R, Tu BP, Cryns VL, Lamming DW, Denu JM. Methyl-Metabolite Depletion Elicits Adaptive Responses to Support Heterochromatin Stability and Epigenetic Persistence. Mol Cell. 2020 Apr 16;78(2):210-223.e8. doi: 10.1016/j.molcel.2020.03.004. Epub 2020 Mar 23. PubMed PMID: 32208170; PubMed Central PMCID: PMC7191556. https://doi.org/10.1016/j.molcel.2020.03.004 Wille CK, Li Y, Rui L, Johannsen EC, Kenney SC. Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency. J Virol. 2017 Mar 1;91(5). doi: 10.1128/JVI.01987-16. Print 2017 Mar 1. PubMed PMID: 28003489; PubMed Central PMCID: PMC5309966. https://doi.org/10.1128/JVI.01987-16 Wille CK, Nawandar DM, Henning AN, Ma S, Oetting KM, Lee D, Lambert P, Johannsen EC, Kenney SC. 5-hydroxymethylation of the EBV genome regulates the latent to lytic switch. Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):E7257-65. doi: 10.1073/pnas.1513432112. Epub 2015 Dec 9. PubMed PMID: 26663912; PubMed Central PMCID: PMC4703011. https://doi.org/10.1073/pnas.1513432112
Education
- BS, Cellular and Molecular Biology, and Genetics, University of Delaware–Newark
- PhD, Microbiology, University of Wisconsin–Madison
Research Description
I am interested in the epigenetic underpinnings of development and disease. In my graduate work, I studied how Epstein-Barr virus co-opts host epigenetic defenses to intricately modulate its life cycle. In my postdoctoral research, I discovered a novel antagonistic feedback loop of H3K79me2, a relatively uncharacterized histone modification enriched on the bodies of expressed genes, and the activating modification H3K9ac. This newly uncovered epigenetic network regulates transition of RNA polymerase II to transcriptional elongation, and enforces maintenance of somatic cell identity.
Affiliations
- International Society for Stem Cell Research (ISSCR)
- American Association for Cancer Research (AACR)
- American Society of Microbiology (ASM)
Honors
- Travel Grant Award from the University of Wisconsin Carbone Cancer Center Heidi Dvinge and Patti Keely Trainee Honor Society (2022)
- Best Poster Award at 16th Wisconsin Stem Cell Symposium, BioPharmaceutical Technical Center Institute (2022)
- Travel Award from the Stem Cell & Regenerative Medicine Fall Conference, Madison, WI (2021)
- Best Poster Award at Stem Cell & Regenerative Medicine Fall Conference, Madison, WI (2021)
- Stem Cell & Regenerative Medicine Center Training Grant (2019)
- Virology Training Grant (2010-2012)
- Shapiro Scholarship for Incoming UW Graduate Students (2009)
- Phi Beta Kappa Honor Society (2009)
- Donald W. Harward Research Fellowship (2009)
- Howard Hughes Medical Institute Research Scholar Summer (2007)
- Beta Beta Beta Biological Honor Society (2006-2009)
- Alpha Lambda Delta Honor Society (2006)