The advent of the CRISPR-CAS9 technique gives researchers the ability to make precise changes to the DNA of, theoretically, any living organism that has DNA. That precision is particularly meaningful for treatment of diseases caused by well-known faults in genes — otherwise incurable diseases such as sickle cell anemia, the fatal lung disorder cystic fibrosis, and the blindness-causing Leber congenital amaurosis. CRISPR-Cas9 could be a way to prevent or reverse those disorders.
An interdisciplinary pair of WID researchers has developed a new nanocapsule delivery method for delivering the CRISPR-Cas9 gene editing tool. The new system could be used for many types of gene therapies.
Human cell engineering including CRISPR gene editing and epigenetic reprogramming
Engineering cells and organism via genome editing and studying the delivery method for CRISPR/Cas9
CRISPR-Cas9 Strategy to Generate DOT1L-Deficient Embryonic Stem Cells to Study Pluripotency
WID’s Randolph Ashton, assistant professor of biomedical engineering, is the new associate director for UW–Madison’s Stem Cell and Regenerative Medicine Center.
The Wisconsin State Journal, as part of its feature highlighting stem cell research at UW 20 years after James Thomson’s discovery, highlights WID researchers Randolph Ashton and Kris Saha.
A second WID-led team joins NIH’s Somatic Cell Genome Editing Consortium with a grant to study new methods of delivering the CRISPR/Cas9 system to the brain.
UW researchers led by WID’s Kris Saha join the National Institutes of Health’s Somatic Cell Genome Editing Consortium with a major collaborative award.
Development of motor neurons derived from human stem cells via sectioning, staining, and microscopy
Lab Manager
Genomic DNA sequencing and viral production. Facilitate various administrative functions.
Investigators from WID are among the recipients of the latest round of UW2020 awards.
Error rates as high as 50 percent are a problem when the goal is to correct typos in the DNA that cause genetic disease. Now, a team of researchers led by WID’s Kris Saha has made the fix less mistake-prone.
Professor
Engineering platforms to regulate human pluripotent stem cell differentiation
The National Science Foundation has awarded nearly $20 million to a consortium of universities to support a new engineering research center that will develop transformative tools and technologies for the consistent, scalable, and low-cost production of high-quality living therapeutic cells. Several WID investigators are collaborators on the project.
In vitro modeling of CAR T cell cytotoxicity, activation, and exhaustion
